What is HEK 293 cells used in?
HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals.
How do HEK293 cells grow?
Growth Conditions: HEK293 cells should be grown in a humidified incubator at 37°C, supplemented with 5% CO2. Storage Conditions: For long-term storage, HEK293 cells should be kept in the liquid nitrogen vapor phase. Keeping them at -80°C is not recommended as it will result in loss of viability over time.
How was HEK293 harvested?
The cells, called HEK 293 cells (that stands for human embryonic kidney) were taken from an aborted fetus in the 1970s in the Netherlands. Bits of chopped up DNA from the adenovirus, a virus that causes a pretty severe cold.
How long does it take for HEK 293 cells to adhere?
HEK-293 cells see cell doubling once every 24–48 hours depending on the care. Most HEK-293T cells double every 12–20 hours depending on conditions. Our cell doubling time is typically closer to 12 hours.
How long do HEK 293 take to adhere?
How long can you passage HEK 293 cells?
Under optimum growth conditions (37°C, 5% CO2), 293 cells double about every 36 hr. To maintain consistency, do not passage cells indefinitely. For best results, we recommend you use low passage 293 cells for transfection and titration procedures.
How is HEK293 immortalized?
The HEK293 cell line was originally immortalized by the random integration of viral genomic DNA of adenovirus 518, which includes the E1A and E1B genes.
Is HEK293 a normal cell line?
HEK293 is embryonic kidney immortalized cell line, which represents the epithelial morphology and possibility to form tumors in nude mice. HEK 293 cells are popular for their ease of growth and transfection, making them a common cell culture in cancer research.
Are HEK 293 immortal?
HEK 293 immortal cell line that is comparatively easy to handle. An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.