What is BG11 medium?
BG11 Broth is a universal medium for the cultivation and maintenance of blue green algae (cyanobacteria).
How to prepare BG11 media?
Combine the following solutions and adjust pH to 7,5 (Use 1.0M HCl or NaOH). Stock Per liter of medium Stock 1 10 ml Stock 2 10 ml Stock 3 10 ml Na2CO3 0,02 g Stock 4 1,0 ml Page 2 NaNO3 1.5 g (For 100x BG-11 solution don’t add NaNO3) For solid media: add 1.5% agar to the liquid BG11 media. 3.
Is nutrient agar a complex or defined medium?
Nutrient Agar Is a Complex Media Since many pathogenic (disease-causing) bacteria fall into the non-fastidious heterotrophic category, a complex media consisting of various nutrients such as peptones and beef extracts is the ideal choice for bacterial growth and cultivation.
What is the use of ferric ammonium citrate?
Ferric citrate is used to control phosphorus levels in adults with chronic kidney disease who are on dialysis. Ferric citrate is also used to treat iron deficiency anemia in adults with chronic kidney disease who are not on dialysis. Ferric citrate may also be used for purposes not listed in this medication guide.
How do you make tap media?
- Add 10 mL 2M Tris base (e.g. Trizma)
- Add 10 mL Solution A.
- Add 1 mL Phosphate solution.
- Add 1 mL Hutner`s trace solution.
- Add 1 mL Glacial acetic acid , then add drops until pH 7 is reached.
- Stop mixing, and add dd H20 until 1 L final volume is reached.
- Start mixing again until complete mixing is achieved.
What type of medium is nutrient agar?
Nutrient agar is a general purpose medium that supports the growth of a wide range of non-fibrous organisms. Nutrient agar is popular because it supports the growth of various types of bacteria and fungi, and contains many of the nutrients necessary for the growth of bacteria.
What is the use of ferric ammonium citrate folic acid and vitamin b12 syrup?
used to treat iron deficiency anaemia and to prevent folic acid deficiency.
Is ammonium ferric citrate safe?
► Ferric Ammonium Citrate can affect you when inhaled. ► Contact can irritate the skin and eyes. ► Inhaling Ferric Ammonium Citrate can irritate the nose, throat and lungs.
What is tap medium?
TRIS acetate phosphate (TAP) medium.. TAP medium is the most widely used for growth of the green alga Chlamydomonas reinhardtii. Note the presence of acetate effects the photosynthetic ability of the alga and minimal medium should be used instead if quantifying effects of mutations or inhibitors on photosynthesis.
How is algae cultured?
A common method of obtaining pure cultures is serial dilution. Cultivators dilute either a wild sample or a lab sample containing the desired algae with filtered water and introduce small aliquots (measures of this solution) into a large number of small growing containers.
What is nutrient medium?
A liquid or gelatinous substance containing nutrients in which microorganisms, cells, or tissues are cultivated for scientific purposes.
How to prepare BG-11 media?
BG-11 medium. Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11o don’t add NaNO3.
What is the volume of BG-11-N medium?
BG-11 (-N) Medium is now available in larger volumes. View the 10-Liter and 100-Liter BG-11 (-N) Media Kits. To approximately 900 mL of dH 2 O add the first 9 components in the order specified while stirring continuously. Bring total volume to 1 L with dH 2 O.
How to prepare bg-11o from noble agar?
Combine stocks and adjust pH to 7.5 (use 1.0N HCl). Aliquot into flasks (50 ml/125 ml flask) with cotton stoppers on top and autoclave. After autoclaving and cooling the pH is about 7.1. For solid media add 1% noble agar. For BG-11o don’t add NaNO3.
What is the best stock solution for BG-11 medium?
BG-11 Medium BG-11 medium A) Stock solutions for BG-11: Stock 1: Na2MG EDTA 0.1g/liter Ferric ammonium citrate 0.6g/liter Citric acid .1H2O 0.6g/liter CaCl2.2H2O 3.6g/liter Filter sterilize into a sterile bottle or autoclave Stock 2: MgSO4 .7H2O 7.5g/liter Filter sterilize into a sterile bottle or autoclave Stock 3: K2HPO4.3H2O 4.0g/liter orK2HPO4