What is a mobility shift assay used for?
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. It is the core technology underlying a wide range of qualitative and quantitative analyses for the characterization of interacting systems.
What does an electrophoretic mobility shift assay EMSA test?
The electrophoretic mobility shift assay (EMSA) is a rapid and sensitive method to detect protein-nucleic acid interactions1–6. It is based on the observation that the electrophoretic mobility of a protein-nucleic acid complex is typically less than that of the free nucleic acid (Fig. 1).
What is a Supershift in EMSA?
definition. The EMSA supershift is a EMSA experiment carried out using a third lane loaded with the radiolabeled nucleic acid, a protein mixture and an antibody for a specific protein. If an extra retardation is observed, this is due to the formation of a larger complex including the antibody.
What does an electrophoretic mobility shift assay EMSA test quizlet?
an electrophoretic mobility shift assay (EMSA) was conducted to check for the binding of TFIIB to a segment of a DNA promoter region.
What is the difference between ChIP and EMSA?
EMSA is a lot easier to perform than ChIP, however ChIP provides data from a cellular system whereas EMSA is completely in vitro. It depends on what you are trying to prove and how much detail you need.
What is a probe EMSA?
The electrophoresis mobility shift assay (EMSA) is a rapid and sensitive method to detect protein–nucleic acid interactions1-6. It is based on the observation that the electrophoretic mobility of a protein–nucleic acid complex is typically less than that of the free nucleic acid.
How is electrophoretic mobility measured?
The electrophoretic mobility of nano-size colloidal particles can be measured rapidly from the Doppler frequency shift of scattered light associated with the electrophoretic light scattering. In this method, a laser light illuminates colloidal particles dispersed in solvent with an applied electric field.
How do you calculate electrophoretic mobility?
The apparent free electrophoretic mobility can be obtained by applying eqn  ( μ ( mm s − 1 ) = h × T max δ ) . The free electrophoretic mobilities of various marker proteins and five different mammalian carbonic anhydrases calculated by these procedures are listed in Table 6.
What technique can be used to determine DNA protein interactions quizlet?
A DNase footprinting assay is a DNA footprinting technique from molecular biology/biochemistry that detects DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage. This makes it possible to locate a protein binding site on a particular DNA molecule.