What are SK-N-SH cells?
Description. SK-N-SH is a neuroblastoma cell line that displays epithelial morphology and grows in adherent culture. Treatment with all-trans-retinoic acid causes these cells to differentiate and adopt a neuronal phenotype, characterized by extensive neurite outgrowth.
How fast do SH-SY5Y cells grow?
Although SH-SY5Y doubling time was not reported specifically, the parental neuroblast-like populations has a doubling time of approximately 27 h and the subclones were reported to have similar doubling times. SH-SY5Y cells are reported to have a growth saturation density of >1 × 106 cells/cm2.
Why are SH-SY5Y cells used?
SH-SY5Y cells are often used as in vitro models of neuronal function and differentiation. They are adrenergic in phenotype but also express dopaminergic markers and, as such, have been used to study Parkinson’s disease, neurogenesis, and other characteristics of brain cells.
What is neuroblastoma cell line?
Neuroblastoma (NB) cell lines are transformed, neural crest derived cells, capable of unlimited proliferation in vitro. These cell lines retain the ability of differentiation into neuronal cell types on treatment with various agents.
How is SY5Y different from SH?
Differentiation of SH-SY5Y cells relies on gradual serum deprivation; the addition of retinoic acid, neurotrophic factors and extracellular matrix proteins; and serial splitting to select for differentiated mature adherent neurons.
Are SH-SY5Y cells neurons?
SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.
What kind of cells are SH-SY5Y?
What are SH-SY5Y neuroblastoma cells?
Description. SH-SY5Y is a thrice-subcloned cell line derived from the SK-N-SH neuroblastoma cell line. It serves as a model for neurodegenerative disorders since the cells can be converted to various types of functional neurons by the addition of specific compounds.
How do you use passage SH SY5Y cells?
2. Passage of SH-SY5Y Maintenance Cultures
- Split maintenance cultures when cells have reached 70-80% confluency, and do not exceed 10 to 15 passages.
- To passage cells from a T-75 flask, aspirate off media, then rinse with approximately 10 ml 1x PBS.
- Aspirate PBS, and then add 2.5 ml 0.05% Trypsin-EDTA (1x).
What are BV 2 cells?
BV-2 is a type of microglial cell derived from C57/BL6 murine. The BV2 cells are immortalized by v-raf/v-myc carrying J2 retrovirus. BV2 express nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level.
Is SK-N-SH a suitable target cell line for transfection?
SK-N-SH has been used as a target cell line in cell mediated cytotoxicity assays and is a suitable transfection host. The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see ATCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine – beta – hydroxylase.
What is the difference between SK-N-MC and SK-N-SH?
The SK-N-SH line was developed by J.L. Biedler and differs from SK-N-MC (see ATCC HTB-10) in that it exhibits a longer doubling time and higher levels of dopamine – beta – hydroxylase. The cell line is hyperdiploid human female (XX), with the modal chromosome number of 47.
How do I get a certificate of analysis for SK-N-SH (htb-11)?
To download a certificate of analysis for SK-N-SH ( HTB-11 ), enter the lot number exactly as it appears on your product label or packing slip. The certificate of analysis for that lot of SK-N-SH ( HTB-11) is not currently available online. Complete this form to request this certificate of analysis.
Do mRNAs affect cell proliferation in SK-N-as and HEK293 cells?
For both SK-N-AS and HEK293 cells, a concentration of up to 100 ng mRNA in 10(5) cells, encoding a nuclear protein with no other function, did not affect the cells. For evaluation of the method, we screened four different human mRNAs, PDG, DFFA, CORT and PEX14, for their ability to affect cell proliferation in these cells.