What is pBK CMV?
The pBK-CMV phagemid vector1 is a cloning vector derived from a high-copy-number pUC-based plasmid. This vector allows expression in both eukaryotic and prokaryotic systems. Eukaryotic expression is driven by the cytomegalovirus (CMV) immediate early promoter in the pBK-CMV phagemid vector.
Does CMV promoter work in E coli?
coli with a reporter system. Only the promoter from the thymidine kinase gene from HSV1 showed no activity, while the polyhedrin promoter from baculovirus, the early immediate CMV promoter, the early SV40 promoter and the 5′ LTR promoter from HIV-1 directed gene expression in E. coli.
What is the origin of the CMV promoter?
Due to strong expression and compatibility with numerous cell types, promoters derived from the enhancer region of the cytomegalovirus (CMV) immediate early (IE) promoter are among the most common for general expression vectors.
How is CMV promoter activated?
CMV promoter is repressed by p53 and activated by JNK pathway.
Is the CMV promoter active in bacteria?
These results indicate that bacterial promoter activity, either within, or upstream of, the CMV promoter caused read-through transcription into the C-fragment gene resulting in bacterial expression.
Does SV40 promoter work in E coli?
How long is the CMV promoter?
Promoters are about 100 to 1000 base pairs long and found upstream of their target genes. The sequence of the promoter region controls the binding of the RNA polymerase and transcription factors, therefore promoters play a large role in determining where and when your gene of interest will be expressed.
When was CMV first discovered?
Intranuclear inclusions typical of cytomegalovirus infections were first noticed in 1881 by German scientists who thought they represented protozoa. After viruses were grown in cell cultures, Weller, Smith and Rowe independently isolated and grew CMV from man and mice in 1956-1957.
What is a CMV vector?
Cytomegalovirus (CMV) has a series of attributes that makes it attractive as a vector for gene transfer. In healthy individuals the course of the CMV infection is usually asymptomatic.
What binds to the CMV promoter?
The CMV promoter and enhancer contains an array of regulatory elements including multiple binding sites for cellular transcription factors, such as ATF/CREB (activating transcription factor/cAMP response element binding protein), NF-κB/Rel, (nuclear factor κB/Rel), SP-1 (specificity protein 1), ELK-1 (ETS transcription …